ABSTRACT
Infectious pustular vulvo vaginitis [IPV] was isolated and identified from cows showing the genital form of infection, located at Dakahlia Governorate. The clinical viral sign included vulvo-vaginitis, abortion, retained fetal membranes and metritis. IPV was isolated from vaginal swabs on MDBK cell line and confirmed by virus neutralization test [VNT]. SNT and ELIZA detected IBR/IPV antibodies in serum and milk samples. SNT and ELISA revealed that 63 [70%] and 71 [78.1%] sera samples were positive respectively while 35 [58.3%] and 40 [66.7%] milk samples were positive respectively. The biochemical changes are more pronounced associated with sera containing high IBR/IPV antibody titer. There were significant increase protein bands with molecular weight 193.87, 163.97, 156.81, 129.70, 105.03, 100.94, 87.74, 74.67, 73.96 KD and significant decrease of protein band with molecular weight [KD] 138.69, 68.55, 66.63, 65.09, 41.36 and 30.39 KD in sera with low, moderate and high IBR/IPV antibody titer associated with several mineral changes including increase magnesium and iron protein binding capacity associated with serum with low IBR/IPV antibodies titer, while moderate IBR/IPV antibodies sera associated with significant increase in total calcium, inorganic phosphorus, total magnesium, iron and iron binding capacity levels and significant decrease in chloride levels, while significant increase in inorganic phosphorus, magnesium, iron and iron binding capacity levels and significant decrease in chloride level ions was determined in serum with high IBR/IPV antibody titer. The increase in IBR/IPV antibody concentration was associated with metabolic changes that lead to an increased individual animal risk of non-pregnancy and increased risk of abortion. All animals imported to Egypt should be free from IBR/IPV infections. IBR/IPV virus as cause of venereal disease must consider. Control programs for detection and removal of IBR/IPV-persisted cattle should be applied in cattle herds all over the country